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The idea is to familiarise them with new methods of study and structuring thought analysis, summary, research, academic writing, etc. The first cohort of high school students was hosted in July of The programme, which also includes a scientific impact assessment component led by the Laboratory for Interdisciplinary Evaluation of Public Policies LIEPP , has appealed to several of our long-standing partners.

It only took one meeting with students from the Premier Campus programme on the magnificent Reims campus to be wholly captivated by the intelligence and wealth of the approach, and above all to be convinced that this was THE equal opportunity programme to support. Finally, the enthusiasm and pride of the participants is—ultimately—the best indicator, and there is no doubt that the programme will have a major impact on the course their lives take.

EDUCATION Innovating for excellence in education The multidisciplinary academic the last six years, SPEAP has education we offer our trained a body of students 13, students is central from both arts and social to our mission, and relies on science backgrounds, who have innovative teaching formats. The programme of our educational model. It combines cooperative projects that open engineering, design, technology up new ways of understanding. Each year it attracts a cohort of around twenty academics anthropologists, sociologists, linguists, philosophers, historians, legal scholars and physicists and artists choreographers, filmmakers, visual artists, architects and designers.

The course is also open to students on internship or who have recently graduated from Sciences Po. At a time strategy. Research conducted when cities are experiencing at Sciences Po covers the main many technology-related social science disciplines of developments, it is important history, political science, law, to analyse the changes this sociology and economics. The academics chair.

This community produces more than scientific publications each year, more than a quarter of which are authored or co- authored by PhD students. Productive collaboration between academic research and business or institutional organisations is also facilitated through the creation of research chairs focused on major contemporary issues.

This partnership affords the Group a better grasp of public policy related to digital technology, and students a better understanding of changes and issues in mass-market retailing. JOHN D. Ways to give For a company You can choose to contribute to the training of your future employees, strengthen your employer brand, support research or fund excellence or merit scholarships. DD By paying your apprenticeship tax, you encourage excellent dual education programmes and support our development.

DD To enhance your employer brand, Sciences Po raises your profile among students through exclusive events as part of a specific partnership, usually established with one of our graduate schools. Sciences Po also welcomes other forms of donation: legacy gifts, life insurance, gifts in kind and gifts of securities stocks, bonds, etc.

Anne-Sophie Luby annesophie. Our team seeks private resources from individual donors, companies or foundations to fund educational projects, research, social responsibility initiatives and campus infrastructure. These projects are all guided by the same principle: advancing the public interest.

Our team strives every day to establish close contact with donors, partners and sponsors so as to best meet their needs and maintain a stable relationship built on shared values. Contact : contact. Persons on this register are entitled to provide payment services within the context of their authorisation.

Bawag P. Linde have Kiosken, Dyre have vej , , Kolding, Denmark. Vesterv an g Kiosk, Vesterv an gsvej 16, , Viborg, Denmark. Alimentation Les Tropiques, Z. Dahak Khalid Av. Leon Blum, , Clermont Ferr an d, Fr an ce. Exo Bel an ge, 35 Blvd. Foch, , Espinay Sur Seine, Fr an ce. Charles De Gaulle, , Coquelles,. Snc Bi an chin, Za Les Javelottes. Place Miterr an d, , Bonson, Loire, Fr an ce.

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The Foundation also holds events. In , Olivier Blanchard, C. It contributes in particular to our social inclusion policy with the award of scholarships and prizes. An annual fundraising gala is held in London, during which the Assia de Juniac Excellence Scholarship and the Roger Seydoux Merit Scholarship are awarded to two students enrolled in one of the London School of Economics-Sciences Po dual degree programmes.

Imagine an diverse uses urban campus of international Here, all members of the stature at the cutting edge Sciences Po community— of educational innovation. The library, digital experimentation centre, A streamlined urban campus incubator and cafeteria interact Campus is organised harmoniously with the old around two hemispheres on buildings.

This campus-agora either side of Boulevard Saint- will engage with the city and Germain, around the Artillerie, the world. A sustainable campus Because Sciences Po is building a campus for the next thirty—or —years, redevelopment of the Artillerie site focuses on creating flexible spaces that can adapt to educational innovation, while making the most of an exceptional heritage.

I think it will transform Sciences Po. Nicolas Seydoux and Gaumont l Mr. Olivier Reza l Mr. Samir Assaf l Mrs. Isabelle Schlumberger l and one anonymous donor. We the Fondation de France for a thank the sponsors, donors and student from the Mediterranean partners who make our projects region. This year, new benefits students with students were admitted through disabilities.

In addition to a tuition fee waiver, scholarships are awarded to deserving students thanks to support from many partners and donors. The scheme is designed to help scholarship high school students prepare effectively for entering higher education, but is not specific preparation for the Sciences Po entrance exam.

The idea is to familiarise them with new methods of study and structuring thought analysis, summary, research, academic writing, etc. The first cohort of high school students was hosted in July of The programme, which also includes a scientific impact assessment component led by the Laboratory for Interdisciplinary Evaluation of Public Policies LIEPP , has appealed to several of our long-standing partners. It only took one meeting with students from the Premier Campus programme on the magnificent Reims campus to be wholly captivated by the intelligence and wealth of the approach, and above all to be convinced that this was THE equal opportunity programme to support.

Finally, the enthusiasm and pride of the participants is—ultimately—the best indicator, and there is no doubt that the programme will have a major impact on the course their lives take. EDUCATION Innovating for excellence in education The multidisciplinary academic the last six years, SPEAP has education we offer our trained a body of students 13, students is central from both arts and social to our mission, and relies on science backgrounds, who have innovative teaching formats.

The programme of our educational model. It combines cooperative projects that open engineering, design, technology up new ways of understanding. Each year it attracts a cohort of around twenty academics anthropologists, sociologists, linguists, philosophers, historians, legal scholars and physicists and artists choreographers, filmmakers, visual artists, architects and designers.

Allergen contact by the oral route might preferentially induce IgG responses. Published by Elsevier Inc. Sensitization rates of causative allergens for dogs with atopic dermatitis: detection of canine allergen-specific IgE. Allergen-specific IgE serology tests became commercially available in the s.

Since then these tests have been widely used to diagnose and treat allergic skin diseases. However, the relationship between a positive reaction and disease occurrence has been controversial. The purpose of this study was to evaluate allergens using a serologic allergy test in dogs with atopic dermatitis AD.

Among the total 92 environmental and food allergens , house dust and house dust mites were the most common. Several allergens including airborne pollens and molds produced positive reactions, and which was considered increasing allergens relating to the climate changes. The presence of antibodies against staphylococci and Malassezia in cases of canine AD was warranted in this study.

Additionally, strong chicken, turkey, brown rice, brewer's yeast, and soybean and weakly rabbit, vension, duck, and tuna positive reactions to food allergens could be used for avoidance and limited- allergen trials. Comparison of serum concentrations of environmental allergen-specific IgE in atopic and healthy nonatopic horses. Allergic responses in humans, horses and other species are mediated by immunoglobulin E IgE antibodies.

Serum testing to detect allergen-specific IgE antibodies has been developed for dogs, cats and horses; this allows for the identification of allergens and determination of appropriate allergen - specific immunotherapies. This study compared serum allergen-specific IgE concentrations in atopic and healthy horses. Allergen-specific IgE serum concentrations were measured in summer seasons of using a monoclonal anti-IgE antibody. A Northern and Central European allergen panel containing mite, insect, mould and plant pollen allergens , including 15 tests of individual allergens and 5 tests of allergen mixtures was used.

The mean allergen-specific IgE concentrations in the atopic and normal horse populations were compared. Among the atopic horses, the strongest positive reactions occurred against the storage mites Tyrophagus putrescentiae and the domestic mite Dermatophagoides farinae. The atopic horses also demonstrated high IgE concentrations against insects, particularly Tabanus sp.

No horses in the atopic group were IgE-negative. Among all mite, insect, mould and some plant allergen groups the differences in mean specific IgE concentrations between allergic and healthy horses were significant. The mean IgE concentrations for most allergen groups were significantly higher in the atopic horses than in the healthy animals.

However, a high incidence of positive reactions was observed in both healthy and allergic horses. Our results showed a high frequency of polysensitization in atopic horses. Microarray evaluation of specific IgE to allergen components in elite athletes. Allergic sensitization and diseases have been reported to have a very high and increasing prevalence in elite athletes. The pattern of positivity towards the components tested differed from subject to subject, even in those with the same sensitization to allergen extract SPT or sIgE.

Wai, Christine Y. Designer proteins deprived of its IgE-binding reactivity are being sought as a regimen for allergen-specific immunotherapy. Although shrimp tropomyosin Met e 1 has long been identified as the major shellfish allergen , no immunotherapy is currently available. In this study, we aim at identifying the Met e 1 IgE epitopes for construction of hypoallergens and to determine the IgE inhibitory capacity of the hypoallergens.

IgE-binding epitopes were defined by three online computational models, ELISA and dot-blot using sera from shrimp allergy patients. Based on the epitope data, two hypoallergenic derivatives were constructed by site-directed mutagenesis MEM49 and epitope deletion MED Nine regions on Met e 1 were defined as the major IgE-binding epitopes. Both hypoallergens MEM49 and MED showed marked reduction in their in vitro reactivity towards IgE from shrimp allergy patients and Met e 1-sensitized mice, as well as considerable decrease in induction of mast cell degranulation as demonstrated in passive cutaneous anaphylaxis assay.

Both hypoallergens were able to induce Met e 1-recognizing IgG antibodies in mice, specifically IgG2a antibodies, that strongly inhibited IgE from shrimp allergy subjects and Met e 1-sensitized mice from binding to Met e 1. These results indicate that the two designer hypoallergenic molecules MEM49 and MED exhibit desirable preclinical characteristics, including marked reduction in IgE reactivity and allergenicity , as well as ability to induce blocking IgG antibodies.

This approach therefore offers promises for development of immunotherapeutic regimen for shrimp tropomyosin allergy. Immunization with Hypoallergens of shrimp allergen tropomyosin inhibits shrimp tropomyosin specific IgE reactivity. Defining thresholds of specific IgE levels to grass pollen and birch pollen allergens improves clinical interpretation. Cut-off values and predictive values are used for the clinical interpretation of specific IgE antibody results.

However, cut-off levels are not well defined, and predictive values are dependent on the prevalence of disease. The objective of this study was to document clinically relevant diagnostic accuracy of specific IgE for inhalant allergens grass pollen and birch pollen based on test result interval- specific likelihood ratios.

Likelihood ratios are independent of the prevalence and allow to provide diagnostic accuracy information for test result intervals. In a prospective study we included consecutive adult patients presenting at an allergy clinic with complaints of rhinitis or rhinoconjunctivitis. The standard for diagnosis was a suggestive clinical history of grass or birch pollen allergy and a positive skin test. We established specific IgE test result interval related likelihood ratios for clinical allergy to inhalant allergens grass pollen, rPhl p 1,5, birch pollen, rBet v 1.

The likelihood ratios for allergy increased with increasing specific IgE antibody levels. The likelihood ratio was specific IgE specific IgE between 0. Test result interval specific likelihood ratios provide a useful tool for the interpretation of specific IgE test results for inhalant allergens. We performed a longitudinal study of patients with ocular allergies who were treated at the Cornea and Immunology and Allergy Department. Patients underwent an ophthalmologic examination to identify their primary presenting signs and symptoms.

The allergy types were divided into 4 groups. We conducted the following laboratory tests and measurements: blood count, eosinophil count, total serum IgE , and specific IgE. Among 61 patients, 16 Mixed dust mites were positive in Dermatophagoides pteronyssinus dp and Dermatophagoides farinae df antigens were positive in Dust mites, D.

The Th2 immune response, culminating in eosinophilia and IgE production, is not only characteristic of allergy but also of infection by parasitic worms helminths. Anti-parasite IgE has been associated with immunity against a range of helminth infections and many believe that IgE and its receptors evolved to help counter metazoan parasites.

Allergens IgE-antigens are present in only a small minority of protein families and known IgE targets in helminths belong to these same families e. During some helminth infection, especially with the well adapted hookworm, the Th2 response is moderated by parasite-expressed molecules. This has been associated with reduced allergy in helminth endemic areas and worm infection or products have been proposed as treatments for allergic conditions. However, some infections especially Ascaris are associated with increased allergy and this has been linked to cross-reactivity between worm proteins e.

The overlap between allergy and helminth infection is best illustrated in Anisakis simplex, a nematode that when consumed in under-cooked fish can be both an infective helminth and a food allergen. Nearly 20 molecular allergens have been isolated from this species, including tropomyosin Ani s 3 and the EF-hand protein, Ani s troponin.

In this review, we highlight aspects of the biology and biochemistry of helminths that may have influenced the evolution of the IgE response. We compare dominant IgE-antigens in worms with clinically important environmental allergens and suggest that arrays of such molecules will provide important information on anti-worm immunity as well as allergy. Agreement between allergen-specific IgE assays and ensuing immunotherapy recommendations from four commercial laboratories in the USA.

Background Canine allergen-specific IgE assays in the USA are not subjected to an independent laboratory reliability monitoring programme. Six comparisons of pairs of laboratories and overall agreement among laboratories were analysed for ungrouped allergens as tested and also with allergens grouped according to reported cross-reactivity and taxonomy. Altogether, Canine allergen-specific IgE assays in the USA are not subjected to an independent laboratory reliability monitoring programme. The aim of this study was to evaluate the agreement of diagnostic results and treatment recommendations of four serum IgE assays commercially available in the USA.

Allergen-specific Immunoglobulin E IgE determination lies at the heart of diagnosis of sensitization to food and other allergens. In the past few years, reporter systems capable of detecting the presence of allergen-specific IgE have been developed by several labs. These rely on humanized rat basophil leukemia cell lines stably transfected with reporter genes such as firefly luciferase.

We also describe optional treatment steps for enveloped virus and complement inactivation. IgE and allergen-specific immunotherapy-induced IgG4 recognize similar epitopes of Bet v 1, the major allergen of birch pollen. Allergen-specific immunotherapy AIT with birch pollen generates Bet v 1- specific immunoglobulin Ig G 4 which blocks IgE-mediated hypersensitivity mechanisms.

Whether IgG 4 specific for Bet v 1a competes with IgE for identical epitopes or whether novel epitope specificities of IgG 4 antibodies are developed is under debate. Bet v 1a- specific monoclonal antibodies inhibited binding of serum IgE and IgG 4 to The similarities of epitopes for IgE and IgG 4 might stimulate the development of epitope- specific diagnostics and therapeutics.

Total and allergen-specific IgE levels during and after pregnancy in relation to maternal allergy. Type 2 T-helper cell Th2 -skewed immunity is associated with successful pregnancy and the ability to easily direct immune responses to a Th2-polarised profile may be an evolutionary benefit.

The Th2-like immunity associated with allergic disease might generate favourable effects for the maintenance of pregnancy, but could also promote development of Th2-like immune responses and allergic disease in the offspring. Specific and total IgE antibody levels were determined by ImmunoCAP technology at five occasions during pregnancy gestational weeks , , 25, 35 and 39 , as well as at 2 and 12 months after delivery.

Thirty-six women without and 20 women with allergic symptoms were included, of whom 13 were sensitised with allergic symptoms and 30 were non-sensitised without allergic symptoms. The levels of total IgE , but not allergen-specific IgE , were increased during early pregnancy when compared to 12 months after delivery in the sensitised women with allergic symptoms, but not in the non-sensitised women without allergic symptoms p IgE levels during early pregnancy only in the sensitised women with allergic symptoms indicates that allergy is associated with an enhanced Th2 deviation during pregnancy.

Total IgE concentrations are higher in dogs than in humans. Persistent Toxocara canis larval infection is prevalent in dogs and is associated with substantial specific antibody reactions. A correlation, however, between total IgE and T.

To determine the relationship between total IgE , T. Sera of 30 atopic and 30 non-atopic client-owned dogs. Total IgE , T. A positive correlation was demonstrated between T. No differences were detected on the basis of age, gender, vaccination status; deworming or season between atopic and non-atopic dogs. Previous immunomodulatory treatment and cause of atopy did not influence antibody levels of atopic dogs.

Toxocara canis- specific IgE appears to be a major component of total IgE in dogs. Total and T. It is speculated that T. Both methods are equally significant in terms of sensitivity and specificity. The nucleotide sequence was amplified using conventional PCR and the bands were visualized on 2. We performed such an interassay comparison using Korean allergic patients with the following: asthma We compared the sIgE detection performance of both systems for 10 major inhalant allergens Dermatophagoides pteronyssinus, Dermatophagoides farinae, Blattela germanica, cat dander, dog hair, alder, birch, oak, ragweed, and mugwort and four food allergens egg white, cow milk, peanut, and shrimp.

Although both assays showed good accuracy in ROC curve analysis, some minor differences were noted. However, we should take into account the intermethod differences between both assays for clinical applications.

We sought to determine the number of CD23 molecules on immune cells in allergic patients and to investigate whether the number of CD23 molecules on antigen-presenting cells is associated with IgE levels and influences allergen uptake and allergen-specific T-cell activation. Numbers of CD23 molecules on immune cells of allergic patients were quantified by using flow cytometry with QuantiBRITE beads and compared with total and allergen-specific IgE levels, as well as with allergen -induced immediate skin reactivity.

Defined IgE- allergen immune complexes were formed with human monoclonal allergen-specific IgE and Bet v 1. Food allergen-specific serum IgG and IgE before and after elimination diets in allergic dogs. Serum food allergen-specific antibody testing is widely offered to identify suitable ingredients for diets to diagnose adverse food reaction AFR in dogs with allergic skin disease.

Antibody concentrations in blood samples obtained during an unsuccessful diet to help in the choice of diet changes may be influenced by the previous diet. The objective of this paper was to measure food antigen- specific IgE and IgG for the most commonly used 16 food antigens before and after an elimination diet.

Dogs had detectable IgE antibodies to beef, pork, lamb and cows' milk; and detectable IgG antibodies to beef, pork, lamb, cows' milk, chicken and turkey. Of 19 dogs with complete data sets, 14 dogs showed clear improvement during diet and in 7 dogs AFR could be diagnosed by deterioration on rechallenge and subsequent improvement on refeeding the diet. Serum was obtained before and weeks after beginning such a diet.

In these 19 dogs in which an elimination diet was used for the diagnosis of food allergy and in which 14 were probably food allergic and 7 were proven food allergic there were no significant differences in food- specific antibodies before and after an elimination diet of weeks. Birch pollen-associated plant food allergy is caused by Bet v 1- specific IgE , but presence of cross-reactive IgE to related allergens does not predict food allergy.

The role of other immunoglobulin isotypes in the birch pollen-plant food syndrome has not been investigated in detail. Immunoglobulins to Gly m 4, Vig r 1 and Api g 1. Measurements of allergen-specific immunoglobulins are not suitable for diagnosing Bet v 1-mediated plant food allergy to hazelnut and Rosaceae fruits.

In contrast, IgE and IgA to the distantly related allergen Api g 1 correlate with allergy to celeriac. ABPA diagnosis in cystic fibrosis patients: the clinical utility of IgE specific to recombinant Aspergillus fumigatus allergens. Allergic bronchopulmonary aspergillosis ABPA is a complicating factor of cystic fibrosis which can result in a devastating combination as lung disease progresses.

The overlap between the signs and symptoms of the two conditions makes diagnosis problematic, even if standardized criteria are used. The objective of this study was to identify, in a group of cystic fibrosis patients, cases of ABPA by assaying IgE specific to recombinant Aspergillus fumigatus antigens and to compare the method with the Cystic Fibrosis Foundation diagnostic criteria. Fifty-four patients, aged 2 to 20 years, presenting characteristics that could occur with ABPA in isolation, were systematically assessed based on the following: clinical data, a chest CT scan, immediate hypersensitivity skin test for A.

Thirty-nine patients were eligible for the study. Thirty-two of these were investigated. Sensitization to A. Both the Cystic Fibrosis Foundation criteria and the recombinant antigen specific IgE assay defined three patients as suffering from ABPA; however, only two of these patients were diagnosed by both methods. The detection of A. Nevertheless, diagnostic confirmation cannot be divorced from clinical findings, and before this method can be used for ABPA diagnosis, for detecting relapses and for defining cure criteria, longitudinal studies with larger numbers of patients are required.

House dust-mite allergen exposure is associated with serum specific IgE but not with respiratory outcomes. Exposure to house dust has been associated with asthma in adults, and this is commonly interpreted as a direct immunologic response to dust-mite allergens in those who are IgE sensitized to house dust-mite. Generalized linear mixed models were employed to explore the association of respiratory symptoms with house dust-mite concentrations, adjusting for individual and household confounders.

There was no overall association of respiratory outcomes with measured house dust-mite concentrations, even in those who reported they had symptoms on exposure to dust and those who had physician-diagnosed asthma. In conclusion, there was no evidence that respiratory symptoms in adults were associated with exposure to house dust-mite allergen in the mattress, but an association of house mite with strong sensitization was observed.

The clinical efficacy of in vitro allergen-specific IgE antibody test in the diagnosis of allergic children with asthma. Asthma is a very common respiratory allergic disease in Taiwan. The aims of this study were to investigate the allergen -sensitized profile and its relationship with serum total IgE levels in allergic asthmatic children in Taiwan. Moreover, the number of allergens to be tested for the most efficient and effective diagnosis of allergic diseases was also examined.

House dust mites Der p Dermatophagoides pteronyssinus , Der f Dermatophagoides farinae , and Bt Blomia Tropicalis had the highest sensitized rates at Candida albican The prevalence and diagnostic value of specific IgE antibodies to inhalant, animal and plant food, and ficus allergens in patients with natural rubber latex allergy. It is well recognised that natural rubber latex allergy can be associated with serological cross-reactivity to plant allergens , especially tropical fruits and Ficus.

In contrast, data on the frequency and clinical value of specific IgE antibodies against these allergens remain rare. In addition, little is known about the prevalence and diagnostic value of specific IgE antibodies to classical inhalant and animal allergens in NRL allergic patients. The purpose of this study was to investigate the prevalence, the sensitivity, and the specificity of these different specific IgE antibodies in patients suffering from NRL allergy.

Serum samples of 42 NRL allergic adults were investigated. All had a history of NRL allergy confirmed by a positive skin test for latex and a positive latex- specific IgE. Samples were analysed for IgE antibodies against 9 plant food allergens avocado, banana, chestnut, fig, kiwi, papaya, peanut, pineapple and tomato and Ficus benjamina. Because double blind placebo-controlled challenges could not be considered, for ethical reasons, patient's food allergy or immediate hypersensitivity for Ficus and inhalant allergens was documented by a standardised questionnaire.

Diagnosis of atopy was based on a relevant history and the presence of a specific IgE antibody to at least one classical inhalant allergen. For some IgE determinations presence or absence of cross-reactivity was investigated by CAP-inhibition tests. Sensitization to food allergens is a common condition in pediatric atopic dermatitis AD.

Recently, the multiple allergen simultaneous test MAST allowing for a comprehensive assessment of atopy has been developed, but the usefulness in young AD children is not known. The study enrolled 50 children up to 2 years old with a diagnosis of AD. Eight percent of children were sensitized to domestic animals cats and dogs.

Almost perfect kappa index 0. The study showed the frequent occurrence of IgE antibodies against food and airborne and animal allergens in young AD children and confirmed the usefulness of MAST-immunoblots for screening of sensitization in pediatric patients. Multiple independent IgE epitopes on the highly allergenic grass pollen allergen Phl p 5.

Group 5 allergens are small proteins that consist of two domains. They belong to the most potent respiratory allergens. To determine the binding sites and to study allergic patients' IgE recognition of the group 5 allergen Phl p 5 from timothy grass pollen using human monoclonal IgE antibodies that have been isolated from grass pollen allergic patients.

Using recombinant isoallergens, fragments, mutants and synthetic peptides of Phl p 5, as well as peptide- specific antibodies, the interaction of recombinant human monoclonal IgE and Phl p 5 was studied using direct binding and blocking assays. Cross-reactivity of monoclonal IgE with group 5 allergens in several grasses was studied and inhibition experiments with patients' polyclonal IgE were performed.

Monoclonal human IgE showed extensive cross-reactivity with group 5 allergens in several grasses. Despite its small size of 29 kDa, four independent epitope clusters on isoallergen Phl p 5. Isoallergen Phl p 5. Inhibition studies with allergic patients' polyclonal IgE suggest the presence of additional IgE epitopes on Phl p 5.

Our results reveal the presence of a large number of independent IgE epitopes on the Phl p 5 allergen explaining the high allergenic activity of this protein and its ability to induce severe allergic symptoms. High-density IgE recognition may be a general feature of many potent allergens and form a basis for the development of improved diagnostic and therapeutic procedures in allergic disease. Background Group 5 allergens are small proteins that consist of two domains.

Objective To determine the binding sites and to study allergic patients' IgE recognition of the group 5 allergen Phl p 5 from timothy grass pollen using human monoclonal IgE antibodies that have been isolated from grass pollen allergic patients. Methods Using recombinant isoallergens, fragments, mutants and synthetic peptides of Phl p 5, as well as peptide- specific antibodies, the interaction of recombinant human monoclonal IgE and Phl p 5 was studied using direct binding and blocking assays.

Results Monoclonal human IgE showed extensive cross-reactivity with group 5 allergens in several grasses. Allergen-specific IgG antibodies purified from mite-allergic patients sera block the IgE recognition of Dermatophagoides pteronyssinus antigens: an in vitro study.

One of the purposes of specific immunotherapy SIT is to modulate humoral immune response against allergens with significant increases in allergen-specific IgG levels, commonly associated with blocking activity. The present study investigated in vitro blocking activity of allergen-specific IgG antibodies on IgE reactivity to Dermatophagoides pteronyssinus Dpt in sera from atopic patients.

Dpt- specific IgG antibodies were purified by ammonium sulfate precipitation followed by protein-G affinity chromatography. The electrophoretic profile of the ammonium sulfate precipitated fraction showed strongly stained bands in ligand fraction after chromatography, compatible with molecular weight of human whole IgG molecule.

This study showed that allergen-specific IgG antibodies purified from mite-allergic patients sera block the IgE recognition of Dermatophagoides pteronyssinus antigens. This approach reinforces that intermittent measurement of serum allergen-specific IgG antibodies will be an important objective laboratorial parameter that will help specialists to follow their patients under SIT.

High correlation of specific IgE sensitization between birch pollen, soy and apple allergens indicates pollen-food allergy syndrome among birch pollen allergic patients in northern China. Birch pollen sensitization and associated pollen-food syndrome among Chinese allergic patients have not been investigated. Sera from allergic patients from the northern part of China and collected during February to July were investigated.

Among the sera, 34 sera Among the 28 sera with Bet v 1- specific IgE , 27 sera Of the 34 Bet v-positive sera, 6 sera Almost all Bet v-positive sera were donated during the birch pollen season. The prevalence of birch allergy among patients visiting health care during pollen season can be as high as The majority of Chinese birch allergic patients are IgE-sensitized to the major birch pollen allergen Bet v 1 as well as to the major apple allergen Mal d 1 and soy bean allergen Gly m 4.

A relatively high number of patients The high prevalence of specific IgE to Mal d 1 and Gly m 4 among Bet v 1-sensitized patients indicates that pollen-food allergy syndrome could be of clinical relevance in China. Effects of routine prophylactic vaccination or administration of aluminum adjuvant alone on allergen-specific serum IgE and IgG responses in allergic dogs.

Eight clinically normal dogs also were vaccinated as a control group. The corn-allergic dogs also had serum immunoglobulin concentrations measured at 8 and 9 weeks after vaccination. Twenty allergic dogs received a s. The allergic dogs were examined during the 8 weeks after aluminum administration for clinical signs of allergic disease.

The allergic dogs had significant increases in serum corn- specific IgE and IgG concentrations 1 and 3 weeks after vaccination but not 8 or 9 weeks after vaccination. Control dogs did not have a significant change in serum immunoglobulin concentrations after vaccination.

After injection of aluminum adjuvant, the allergic dogs did not have a significant change in serum immunoglobulin concentrations or clinical signs. Allergen-specific IgE and IgG concentrations increase after prophylactic vaccination in allergic dogs but not in clinically normal dogs. Prophylactic vaccination of dogs with food allergies may affect results of serologic allergen-specific immunoglobulin testing performed within 8 weeks after vaccination.

Asp f6, an Aspergillus allergen specifically recognized by IgE from patients with allergic bronchopulmonary aspergillosis, is differentially expressed during germination. Aspergillus fumigatus is a pathogenic mould causing allergic and invasive respiratory diseases. Aspergillus allergen Asp f6 is recognized by IgE from ABPA patients, but not from sensitized individuals, a fact that can be used to differentiate between these two groups of allergic patients.

Proteins from hyphae, resting and germinating conidia of A. Recombinant A. A hyphae- specific 23 kDa A. Differential expression of MnSOD was confirmed by immunoblot using a specific mab. In contrast, Asp f8 another intracellular, but not ABPA- specific allergen , was detected in hyphae and conidia. Aspergillus fumigatus is able to colonize its environment by the formation of hyphae.

Hyphae are found in the lung of ABPA patients, but not in patients suffering from atopic asthma. Comparison of the results of intradermal test reactivity and serum allergen-specific IgE measurement for Malassezia pachydermatis in atopic dogs. Malassezia pachydermatis is part of the normal flora of canine skin.

Malassezia hypersensitivity is recognized as a trigger for clinical signs of atopic dermatitis AD in some dogs. Determinations of Malassezia hypersensitivity are often made with intradermal testing IDT , which may have limited availability in a first-opinion veterinary practice.

The purpose of this study was to compare immediate IDT reactivity to M. Eighty-four dogs with a clinical diagnosis of AD. Multi- allergen IDT was performed on all dogs. Serum testing for allergen-specific IgE against a panel of common environmental allergens and M. A receiver operating characteristic ROC curve was used to analyse the results of the two tests.

Although correlation with a clinical diagnosis of Malassezia dermatitis was not attempted in this study, the results indicate that the ELISA may be used to demonstrate the presence of immediate-type Malassezia hypersensitivity in dogs with AD. Vig r 6, the cytokinin- specific binding protein from mung bean Vigna radiata sprouts, cross-reacts with Bet v 1-related allergens and binds IgE from birch pollen allergic patients' sera.

Birch pollen associated allergy to mung bean sprouts is caused by cross-reactivity between the birch pollen allergen Bet v 1 and the mung bean allergen Vig r 1. Bet v 1, Gly m 4, Vig r 1, and Vig r 6 were produced in Escherichia coli.

Bet v 1 completely inhibited IgE binding to both mung bean allergens. Vig r 6 showed partial cross-reactivity with Vig r 1 and activated basophils sensitized with mung bean allergic patients' sera. We demonstrated IgE cross-reactivity despite low sequence identity between Vig r 6 and other Bet v 1-related allergens. Thus, IgE binding to Vig r 6 may contribute to birch pollinosis-associated mung bean sprout allergy.

KGaA, Weinheim. Scope Birch pollen associated allergy to mung bean sprouts is caused by cross-reactivity between the birch pollen allergen Bet v 1 and the mung bean allergen Vig r 1. Conclusion We demonstrated IgE cross-reactivity despite low sequence identity between Vig r 6 and other Bet v 1-related allergens.

The linear range for BLG detection was BLG content in dairy samples was determined, and there was a good agreement between this immunoassay and reversed-phase high-performance liquid chromatography with high recovery. Additionally, BLG content in food samples had an average recovery of Allergenic residues were also detected in hydrolyzed infant formulas.

The method developed could be a practical approach to determine BLG and its allergenic residues in food with a high degree of sensitivity, reliability and recovery. Serum concentrations of allergen-specific IgE in horses with equine recurrent airway obstruction and healthy controls assessed by ELISA. Equine recurrent airway obstruction RAO , also known as heaves, is one of the most common respiratory problems in older horses.

When RAO-affected horses stay pastured or in a dust-free environment for a prolonged time, clinical signs as well as airway inflammation wane. A number of environmental, immunologic, infectious, and genetic factors play an important role in the pathogenesis of RAO, and the immunologic basis of this disease is still poorly understood.

The aim of this study was to investigate the concentrations of allergen-specific IgE in the serum of horses suffering from RAO and healthy controls. The study included a group of 14 adult Polish Konik horses, kept in a standardized environment, and divided into 2 groups: 7 horses which did not have any respiratory problems comprised the control group and 7 horses with a history of RAO constituted the study group. A clinical and laboratory evaluation, endoscopic examination, and bronchoalveolar lavage BAL were performed in all horses.

Sera of all horses were tested against allergens from 9 molds and 3 mites using the Heska Allercept assay. In the serologic tests, a statistically significant difference between both groups was found for specific IgE against mites, wherein Tyrophagus putrescentia correlated most clearly with RAO. There was no difference between groups for IgE specific against molds.

IgE reactivity to hen egg white allergens in dogs with cutaneous adverse food reactions. Egg white, which is majorly composed of ovomucoid, ovalbumin, ovotransferrin, and lysozyme, is a food allergen in dogs. Information of the IgE reactivity to purified egg white allergens supports accurate diagnosis and efficiency treatment in humans. However, to the best of our knowledge, there have been no studies on the IgE reactivity to purified egg white allergens in dogs. We then used sera from the eight dogs with positive IgE reactivity to crude egg white to examine the IgE reactivity to four purified allergens , ovomucoid, ovalbumin, ovotransferrin, and lysozyme, by ELISA.

Moreover, validating these results, the immunoblot analyses were performed using the sera of the three dogs showing the highest IgE reactivity to crude egg white. Both anti-ovomucoid and anti-ovalbumin IgE were detected in the sera of these dogs, while anti-ovotransferrin IgE was not detected. Considering these, ovomucoid and ovalbumin appears to be the major egg white allergens in dogs with CAFR.

Comparison of the efficacy and safety of pollen allergen extracts using skin prick testing and serum specific IgE as references. Allergen extracts may be different due to the difference in dissemination of allergen -containing species in various geographical areas. Therefore, we wish to develop our own extracts to ensure the precision and quality of diagnosis.

To compare the efficacy and safety of our locally prepared pollen allergen extracts to imported ones, using skin prick testing SPT and serum specific IgE sIgE as references. This prospective, randomized, double-blinded, self-controlled study was performed in respiratory allergic adult volunteers who are sensitized to at least one kind of pollen. Each subject was pricked with our Bermuda grass, Johnson grass and careless weed pollen allergen extracts, and also with the imported ones.

In 68 volunteers, our Bermuda, Johnson and careless weed extracts showed No adverse reaction was found in all procedures. There was no significant difference in mean wheal diameter MWD yielded from using local and imported extracts. Significant correlation was found between MWDs of imported pollen extracts and serum sIgE levels p allergen extracts was found. Ovomucoid Gal d 1 specific IgE detected by microarray system predict tolerability to boiled hen's egg and an increased risk to progress to multiple environmental allergen sensitisation.

Egg allergy is a very common finding in early childhood. Detecting hen's egg HE allergy outgrowing and reintroduction of food containing egg is a task for the allergist. We sought to evaluate the suitability of boiled egg food challenge compared with IgE to allergenic molecules from HE white using a microarray system. Sixty-eight children referring to our centre by the family paediatricians for a suspected egg allergy were enrolled.

Patients underwent double-blind, placebo-controlled food challenge with boiled and raw eggs. Challenge outcomes were compared with skin tests performed using egg white and yolk commercial extracts, to prick-prick test with boiled and raw egg white and yolk, total IgE , egg white specific IgE detected using ImmunoCAP and IgE to egg allergens available on the immunosolid phase allergen chip ISAC microarray.

Efficiency analysis was carried out using both raw and boiled egg challenges as gold standard. None of the other tests was able to discriminate patients' response to HE challenge. Furthermore, Gal d 1 positivity seems to lead to broader environmental allergen IgE sensitization. Gal d 1 positive children have a high frequency of HE allergy, whereas Gal d 1 negative children have a high frequency of tolerance to boiled egg. Multiple specific IgE detection by means of ISAC improves the diagnostic approach in HE allergic children, disclosing other food and inhalant allergic sensitizations, anyhow requiring a comprehensive clinical evaluation.

Effects of geohelminth infection and age on the associations between allergen-specific IgE , skin test reactivity and wheeze: a case-control study. Background Most childhood asthma in poor populations in Latin America is not associated with aeroallergen sensitization, an observation that could be explained by the attenuation of atopy by chronic helminth infections or effects of age. Objective To explore the effects of geohelminth infections and age on atopy, wheeze, and the association between atopy and wheeze.

Methods A case-control study was done in subjects cases and controls aged 7—19 years living in rural communities in Ecuador. Wheeze cases, identified from a large cross-sectional survey, had recent wheeze and controls were a random sample of those without wheeze.

Geohelminth infections were measured in stools and anti-Ascaris IgE in plasma. Results The fraction of recent wheeze attributable to anti-Ascaris IgE was The association between atopy and wheeze was greater in adolescents than children. Although Anti-Ascaris IgE was strongly associated with wheeze adj. Our data provided only limited evidence that active geohelminth infections attenuated the association between atopy and wheeze in endemic areas or that age modified this association.

The role of allergic sensitization to Ascaris in the development of wheeze, independent of atopy, requires further investigation. Evidence-based guidelines for anti-allergic drug withdrawal times before allergen-specific intradermal and IgE serological tests in dogs.

Anti-allergic drugs e. Anti-allergic drugs have the potential to influence the results or interpretation of these tests. Three citation databases and abstracts from international meetings were searched for relevant studies. Studies were grouped based on similar interventions and types of tests. Withdrawal times for each type of drug and test were then extrapolated from the study results.

Owing to a lack of studies, recommendations for withdrawal times before ASIS tests cannot be made for topical glucocorticoids and antihistamines. These proposed withdrawal times are based on the existing evidence at the end of Patch testing and allergen-specific serum IgE and IgG antibodies in the diagnosis of canine adverse food reactions. Adverse food reaction AFR is a common differential diagnosis for pruritic dogs. The only way to diagnose AFR is an elimination diet of weeks with a protein and a carbohydrate source not previously fed.

In humans, patch testing has been shown to be a useful tool to diagnose food allergies. In veterinary medicine, serum food allergen-specific antibody testing is widely offered to identify suitable ingredients for such diets. The aim of this study was to determine sensitivity, specificity , negative and positive predictability of patch testing with and serum antibody testing for a variety of common food stuffs. Twenty-five allergic dogs underwent an elimination diet and individual rechallenge with selected food stuffs, food patch testing and serum testing for food-antigen specific IgE and IgG.

Eleven clinically normal control dogs only were subjected to patch and serum testing. The sensitivity and specificity of the patch test were For IgE and IgG the sensitivity was 6. Based on these results, a positive reaction of a dog on these tests is not very helpful, but a negative result indicates that this antigen is tolerated well. We conclude that patch testing and to a lesser degree serum testing can be helpful in choosing ingredients for an elimination diet in a dog with suspected AFR.

Published by Elsevier B. Quantitative measurement of IgE antibodies to purified allergens using streptavidin linked to a high-capacity solid phase. Commercially available assays for IgE antibody provide results in international units per milliliter for many allergen extracts, but this is not easily achieved with purified or novel allergens.

To develop assays for IgE antibody suitable for purified or novel allergens by using a commercially available immunosorbent. Streptavidin coupled to a high-capacity immunosorbent CAP was used to bind biotinylated purified allergens from mite Der p 1 and Der p 2 , cat Fel d 1 , and dog Can f 1. Assays for IgE antibody to these allergens were performed on sera from children asthma and control as well as adults with atopic dermatitis.

The results were validated by serial dilution of sera with high and low levels of IgE antibody and were quantitated in international units per milliliter by using a standard curve. The results provide evidence about the high quantities of IgE antibody to purified inhalant allergens in patients with atopic dermatitis. In addition, the results demonstrate major differences in IgE antibodies specific for mite and cat allergens among children with high exposure to both allergens.

Natural clinical tolerance to peanut in African patients is caused by poor allergenic activity of peanut IgE. In Africa, peanuts are frequently consumed, but severe allergic reactions are rare. We investigated immunological patterns of clinical tolerance to peanut in peanut-sensitized but asymptomatic patients from central Africa compared to peanut-allergic and peanut-sensitized but asymptomatic patients from Sweden.

IgE to Ara h 2 peptide epitopes was analyzed, and allergenic activity was assessed by basophil activation assay. Forty-six percent of the African and all peanut-allergic Swedish patients showed IgE toward one of the highly allergenic peanut allergens Ara h , 6, 9.

IgG and IgG4 specificities and levels could not discriminate between the African asymptomatic and Swedish peanut-allergic patients. Asymptomatic patients almost lacked IgE to Ara h 2 peptides, which were recognized by peanut-allergic patients. Peanut IgE from peanut asymptomatic patients showed poor allergenic activity compared with IgE from peanut-allergic patients. Natural clinical tolerance to peanut in the African patients can be caused by IgE to low allergenic peanut components and by poor allergenic activity of peanut- specific IgE.

High-resolution crystal structure and IgE recognition of the major grass pollen allergen Phl p 3. Group 2 and 3 grass pollen allergens are major allergens with high allergenic activity and exhibit structural similarity with the C-terminal portion of major group 1 allergens. In this study, we aimed to determine the crystal structure of timothy grass pollen allergen , Phl p 3, and to study its IgE recognition and cross-reactivity with group 2 and group 1 allergens.

The three-dimensional structure of Phl p 3 was solved by X-ray crystallography and compared with the structures of group 1 and 2 grass pollen allergens. Cross-reactivity was studied using a human monoclonal antibody which inhibits allergic patients' IgE binding and by IgE inhibition experiments with patients' sera.

Phl p 3 showed IgE cross-reactivity with group 2 allergens but not with group 1 allergens. SPADE identified two conformational IgE epitope-containing areas, of which one overlaps with the epitope defined by the monoclonal antibody. The mutation of arginine 68 to alanine completely abolished binding of the blocking antibody. This mutation and a mutation of D13 in the predicted second IgE epitope area also reduced allergic patients' IgE binding. Group 3 and group 2 grass pollen allergens are cross-reactive allergens containing conformational IgE epitopes.

They lack relevant IgE cross-reactivity with group 1 allergens and therefore need to be included in diagnostic tests and allergen-specific treatments in addition to group 1 allergens. Correlation between skin-prick testing, individual specific IgE tests, and a multiallergen IgE assay for allergy detection in patients with chronic rhinitis.

Allergy test results can differ based on the method used. The most common tests include skin-prick testing SPT and in vitro tests to detect allergen-specific IgE. This study was designed to assess allergy test results using SPT, individual specific IgE tests, and a multiallergen IgE assay multiple allergen simultaneous test in patients with chronic rhinitis and controls.

One hundred forty total patients were prospectively enrolled in the study, including patients with chronic rhinitis and 40 control patients without atopy. All eligible patients underwent SPT, serum analysis using individual specific IgE test, and multiple allergen simultaneous test against 10 common allergens. Allergy test results were then compared to identify correlation and interest agreement.

Individual specific IgE test and multiple allergen simultaneous test allergy detection prevalence was generally similar to SPT in patients with chronic rhinitis. Agreement and correlation between individual specific IgE test and multiple allergen simultaneous test were good to excellent for a majority of tested allergens. This study shows good agreement and correlation between SPT with individual specific IgE test and multiple allergen simultaneous test on a majority of the tested allergens for patients with chronic rhinitis.

Comparing the two in vitro tests, individual specific IgE test agrees with SPT better than multiple allergen simultaneous test. Skin prick test responses and allergen-specific IgE levels as predictors of peanut, egg, and sesame allergy in infants. Ninety-five percent positive predictive values PPVs provide an invaluable tool for clinicians to avoid unnecessary oral food challenges.

HealthNuts is a population-based, longitudinal food allergy study with baseline recruitment of 1-year-old infants. Any infant with a detectable SPT response was invited to undergo oral food challenge and sIgE testing. Five thousand two hundred seventy-six infants participated in the study. Results were robust when stratified on established risk factors for food allergy. All data resulting from this study will be catalogued in SDAP. An assay that may predict the development of IgG enhancing allergen-specific IgE binding during birch immunotherapy.

Background It has been shown that birch pollen immunotherapy can induce IgG antibodies which enhance IgE binding to Bet v 1. We aimed to develop a serological assay to predict the development of antibodies which enhance IgE binding to Bet v 1 during immunotherapy. Blocking and possible enhancing effects on IgE binding were compared with skin sensitivity to Bet v 1 after treatment. Results We found that fragment- specific IgG enhanced IgE binding to Bet v 1 in two patients who also showed an increase of skin sensitivity to Bet v 1.

Conclusion Our results indicate that it may be possible to develop serological tests which predict the induction of unfavourable IgG antibodies enhancing the binding of IgE to Bet v 1 during immunotherapy. Identification of Aspergillus A. Aspergillus species A. Current diagnostic modalities employ crude Aspergillus extracts which only indicate the source to which the patient has been sensitized, without identifying the number and type of allergens in crude extracts.

We report a study on the identification of major and minor allergens of the two common airborne Aspergillus species and heterogeneity of patients' IgE response to them. Corresponding EAST positivity was In immunoblots, 5 allergenic proteins were identified in A. Twelve proteins bound patients' IgE in A. The position and slopes of EAST binding and inhibition curves obtained with individual sera varied from patient to patient. The number and molecular weight of IgE-binding proteins in both the Aspergillus extracts varied among patients.

This approach will be helpful to identify disease eliciting molecules in the individual patients component resolved diagnosis and may improve allergen-specific immunotherapy. IgE sensitization to food allergens and airborne allergens in relation to biomarkers of type 2 inflammation in asthma.

We have recently reported that sensitization to food allergens and sensitization to airborne allergens had independent associations with increased fraction of exhaled nitric oxide FeNO and blood eosinophils in middle-aged adults and in young subjects with asthma. To investigate the relation between IgE sensitization and several type 2 inflammation biomarkers in adult asthmatics. Asthmatics sensitized to food allergens had higher FeNO, Assessing the profile of allergic sensitization, including to food allergens , might improve the understanding and.

IgE binding to peanut allergens is inhibited by combined D-aspartic and D-glutamic acids. The objective of this study was to determine if D-amino acids D-aas bind and inhibit immunoglobulin E IgE binding to peanut allergens. Each sample was mixed with a pooled plasma from peanut-allergic donors, and tested by ELISA enzyme-linked immunosorbent assay and Western blots for IgE binding to peanut allergens. Published by Elsevier Ltd.

IgE-ScFvs reacted with recombinant Phl p 1 and natural group 1 grass pollen allergens. Using synthetic Phl p 1—derived peptides, the binding sites of two ScFvs were mapped to the N terminus of the allergen.

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